The Immunology/Hereditary Autoinflammatory Diseases Scientist must demonstrate experience and understanding of techniques directly applicable to tissue culture, molecular and cellular biological techniques such as PCR, flow cytometry, IHC, etc. Upon contract award, the company is seeking an individual that meets or exceeds these requirements:

Work Location:

Bethesda MD, 20892. No remote work.

Roles and Responsibilities:

• Provide Scientific Research Support – The individual shall drive discovery and translational research aimed at identifying, characterizing, and interpreting results for novel diagnosis for inflammatory diseases.
• The individual must be able to create their own research plans with minimum support from the principal investigator. The individual must be able to run research plans independently. Weekly/as needed meetings will be held to evaluate the work and provide directions for next steps.
• Provide Scientific Research Implementation – The individual shall define and implement critical studies needed to support hypothesis-based research. The individual ‘s progress will be parodically evaluated and the individual will be provided with feedback and directions.
• Provide Scientific Publication Support – The individual shall make contributions to scientific literature and conferences through publication and presentation of research results. The individual will be evaluated based on preparing manuscripts and abstracts for scientific publications.
• Provide Identify/Design/Develop In-Vitro Assays – The individual shall identify and evaluate a wide variety of in vitro assays to test immunological, metabolic, and various disease mechanisms.
• The individual shall contribute to in-vitro assay design, optimization, and derive actionable biological conclusions. The individual shall perform assay development, qualification and validations as needed.
• Provide Cell-Based Assays – The individual shall conduct cell-based assays including, but not limited to, ELISA and Flow Cytometry.
• Provide Blood Cell Services – The individual shall separate, isolate and cryopreserve primary cells peripheral blood mononuclear cells (PBMCs).
• Provide Data Organization – The individual shall organize data prior to scientific publication submission. The data will be reviewed and evaluated by the principal investigator.
• Provide Lab Maintenance/Safety – The individual shall ensure lab maintenance activities and equipment calibration are performed according to schedule.
• The individual shall ensure lab maintenance activities and maintain a clean and safe lab environment.

Qualifications:

• PhD with 15+ years of directly relevant experience in immunology, or inflammation.
• Proficiency in T cell culture, multicolor Flow Cytometry, RNA-seq, western blotting, q-PCR, multiplex ELISA, RNA/DNA isolation, T cell isolation, cell assay development, mouse handling/ organ isolation is required.
• Expertise in lentiviral production, synthetic biology, lentiviral vector design desired.
• Expertise in exosome purification and characterization.
• Knowledge of standard Human T cell isolation & in vitro culture techniques is required.
• Possess experience using autoimmune and inflammatory disease mouse and rabbit models.
• Good understanding of immune signaling pathways and interest in metabolomics.
• Ability to contribute creatively and strategically to in vitro assay design, optimization and derive actionable biological conclusions.
• Ability to define and implement critical studies needed to support hypothesis-based research.
• Ability to write protocols and study reports, produce and maintain accurate records.
• Expertise with data analysis software such as Excel and Graphpad Prism
• Strong verbal and written communication skills.
• Ability to synthesize complex scientific problems into a strategy and tactics.
• Familiarity with both Microsoft and Mac operating systems, including PowerPoint, Excel, Word, Adobe Photoshop and Access.
• Excellent interpersonal, analytical, organizational and time management skills.

Competencies Needed:

• ·Tissue processing and purification of immune cells, in vitro cell based bioassay development, tissue cell culture (primary mouse and human cells, cell lines and genetically engineered cell lines)
• Multicolor flow cytometry (FACS), and ELISA assays
• Basic molecular biology techniques (cloning, viral tranductions, etc.)
• Analyze biological data using FlowJo, Microsoft Excel, and GraphPad Prism
• Interpret, analyze and present data; work in fast-paced, result oriented research environment
• Contribute to project advancement by developing new methods or technologies and troubleshooting existing protocol
• Write protocols and study reports, produce and maintain accurate records
• Prepare manuscripts for journal publications

Job Type: Full-time

Pay: $73,414.83 - $104,240.49 per year

Benefits:

• Health insurance
• Paid time off

Schedule:

• 8 hour shift
• Day shift
• Monday to Friday

COVID-19 considerations:
Mandatory COVID-19 Vaccination Requirement

Ability to commute/relocate:

• Bethesda, MD 20892: Reliably commute or planning to relocate before starting work (Required)

Education:

• Doctorate (Required)

Experience:

• Cell-Based Assays: 10 years (Required)
• Identify/Design/Develop In-Vitro Assays: 10 years (Required)
• Scientific Research Implementation: 10 years (Required)
• exosome purification and characterization: 10 years (Required)
• lentiviral production, lentiviral vector design: 5 years (Preferred)
• Lab Maintenance/Safety: 10 years (Preferred)

Language:

• English (Required)

Work Location: Hybrid remote in Bethesda, MD 20892

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